Vigilancia Tecnológica

Disclosed herein is a microfiltration device comprising a microfilter coated with a positively charged degradable layer and a method of preparing the microfiltration device. In particular, the positively charged degradable layer comprises a polymer substrate and a cation, and wherein the polymer substrate is alginate gel and the cation is calcium (II). Also disclosed is a method of separating a biological material from a sample using said microfiltration device and a kit comprising said microfiltration device. In particular, the biological material is a negatively charged bacteria, fungus, or virus. The microfiltration device and method of separating a biological material described herein demonstrate good compatibility with various downstream analysis techniques.