This paper proposes a series of experimental determinations carried out with the aim of generating new conclusions regarding the ability of 3D-printed gears to be integrated into mechanisms without lubrication. The main factors that influence the appearance of wear in non-lubricated contact are sliding speed, material hardness, surface finish, surface geometry, and material microstructure. The tests considered the type of material from which they were made and the 3D printing technology type. For testing the gear wheels, a mechatronic experimental setup was made consisting of two shafts with adjustable axial distances, a wheel loading system gears, an electric motor, and a command-and-control system. In terms of materials, four types of materials were monitored: PA (polyamide), PLA (polylactic acid), ABS (acrylonitrile butadiene styrene) and PP (photopolymer). The evaluation of the gear wear was carried out by checking the gearing on two flanks (Frenco ZWP 06) and by scanning with the ATOS CORE 135 3D scanner. The PA and PP gears failed to meet the structural integrity standards after the tests. The PLA gears exhibited superior resistance to abrasive wear compared to the ABS gears, whereas the ABS gears generally demonstrated stronger structural integrity.