Vigilancia Tecnológica

The invention provides methods and device for determining the identity and order of units of large biopolymers. Device of the invention use electrical fields to translocate the molecule through the channel and pass the molecule, base-by-base, by an enhancement structure that enhances an incoming optical stimulus such as an electromagnetic field or wave. The presence of a DNA bases at the exit, adjacent the enhancement structure, has a characteristic effect in response to the enhanced optical stimulus, such as a characteristic fluorescence or Raman scattering. The characteristic effect of each base is read by the detector to determine the sequence of the molecule.