Vigilancia Tecnológica

Described herein are methods and systems useful to analyze proteins/polypeptides, including sequentially sequencing individual polypeptides, as well as compounds useful in carrying out such methods. Such compounds include those depicted in Formulas (I)-(XX). In embodiments, the methods involve expanding the distance between each amino acid of a polypeptide chain by transferring individual amino acids, in order, from a donor polypeptide to a polymer (e.g., a nucleic acid backbone) to form a hybrid polymer (a PROSE construct), then sequencing the individual amino acids along the PROSE construct. Such sequencing may involve nanopore-type sequencing, SMRT-sequencing, and the like.