Vigilancia Tecnológica

The present invention provides a method for simultaneously performing cell culture and exosome isolation and purification by using an aqueous two-phase system during plant cell culture. Exosome production and isolation can be simultaneously performed thereby during culturing. In addition, when an aqueous two-phase system is used, concentration into a dextran layer occurs such that process efficiency can become far higher than that of the isolation of exosomes secreted in a culture solution after conventional culturing. In the maintenance of the stability and function of produced exosomes, the present invention selects polymers PEG and dextran as constituent materials for the formation of an ideal aqueous two-phase system having a low concentration, and thus has excellent efficiency.